HA-tagged Protein Purification...
- Article
- HA-tagged Protein Purification Gel
- Product Number
- 3321
- Supplier
- MBL International
- Size
- 1 ml
- Description
- The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require highly purified, active proteins for studies involving signaling pathways, enzymology, receptor binding, DNA binding, post-transcriptional modifications, and much more. Thus, choosing a method of purification is an important aspect in maintaining protein structure and function. Recombinant tagged protein purification methods and kits are now widely recognized. The HA epitope tag, YPYDVPDYA, is derived from Human influenza hemaglutinin (HA) 98-106 aa. This HA-tag is also commonly used in such as mammalian cell expression vectors. MBLI's HA-tagged Protein Purification Gel is designed for the isolation of HA-tagged protein from cell culture supernatants and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. Therefore, MBLI has developed the Anti-HA-tag Gel to purify HA-tagged proteins quickly and efficiently at neutral pH to maintain the activity and conformation of purified proteins. The elution of HA-tagged proteins from the Gel is achieved by the addition of the HA-tag peptide (YPYDVPDYA). As the HA-tag peptide competes with HA-tagged proteins on the Gel, the purified proteins do not lose the protein activity.
- Category
- Protein Purification & Analysis
- Product Group
- Precast Gels
- Shipping Temperature
- BLUE ICE
- Supplier URL
- Visit supplier product page