Anti CD19 CAR Lentivirus (CD19...

Anti CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3z)

78600

BPS Bioscience

50 ul

The anti-CD19 CAR lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express the ScFv portion of anti-CD19 (clone FMC63) linked to 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge, 4-1BB, and CD3ζ signaling domains.

Cell Culture

Primary Cells & Cell Lines

The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM. Media Formulation Recommended CD4+CD8+ T Cell Medium: StemSpan SFEM (Stemcell Technologies, #09650) supplemented with 10% heat-inactivated FBS (Life Technologies, #10082147), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01), plus 10 ng/ml IL-2 (BPS Bioscience, #90184).

DRY ICE