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Direct-zol RNA MiniPrep w/ Zym...

Article
Direct-zol RNA MiniPrep w/ Zymo-Spin IIC Columns (Capped)
Product Number
R2052
Supplier
Zymo Research
Size
200 preparations
Description
Isolate total RNA and small/miRNA from TRIzol® without phase separation|The Direct-zol™ RNA MiniPrep Kits provide a streamlined method for the purification of up to 50 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.
Category
Molecular Biology
Product Group
DNA Purification
Shipping Temperature
RT
Supplier URL
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