Flipper-TR™ is a fluorescent probe that specifically targets the plasma membrane of cells and reports membrane tension changes through its fluorescence lifetime changes. It is the most advanced member of the Flipper probes family, which sense changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Flipper-TR™ spontaneously inserts into the plasma membrane of cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. A detailed protocol for staining and FLIM imaging can be found in the datasheet. Flipper-TR™ works on a wide range of organisms including plants, gram positive bacteria, yeast and mammalians.
