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Mouse TrueBlot ULTRA: Anti Mou...

Article
Mouse TrueBlot ULTRA: Anti Mouse Ig HRP
Product Number
18-8817-33
Supplier
Rockland Immunochemicals
Size
200 ul
Description
Mouse IgG TrueBlot® ULTRA is a unique Anti-Mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blot data with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Mouse IgG TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
Category
Antibodies
Product Group
Secondary Antibodies
Application
Bioact, ChIP, ELISA, IF, IP, WB
Host
Rat
Species reactivity
Mouse
Conjugate
Horseradish Peroxidase
Clone ID
eB144
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Shipping Temperature
BLUE ICE
Supplier URL
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