Mouse TrueBlot ULTRA: Anti Mou...

Article: Mouse TrueBlot ULTRA: Anti Mouse Ig HRP
Product Number: 18-8817-33
Supplier: Rockland Immunochemicals
Size: 200 ul
Category: Antibodies
Product Group: Secondary Antibodies
Application: Bioact, ChIP, ELISA, IF, IP, WB
Host: Rat
Species reactivity: Mouse
Conjugate: Horseradish Peroxidase
Clone ID: eB144
Shipping Temperature: BLUE ICE
Supplier URL: Visit supplier product page

Mouse TrueBlot® IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.

Size: 200 ul

358.00 CHF