PKmito Orange FX (also known as PKMO FX) is a bright, photostable, mitochondrial probe based on the PKmito™ dyes developed in the lab of Zhixing Chen at Peking University. PKmito Orange FX labels mitochondria in formaldehyde or glutaraldehyde fixed cells with very high specificity. The unique and unmatched feature of PKmito Orange FX is its ability to be retained nearly quantitatively after glutaraldehyde fixation of stained cells. PKmito Orange FX is extremely well suited for STED and SIM superresolution imaging. It allows to perform nanoscopy of mitochondria with exquisite resolution and bright signal. PKmito Orange FX accumulates in the mitochondrial inner membrane (IM) and stays in place upon fixation. It is highly suited to image mitochonridal cristae structure by STED superresolution microscopy using a 775 nm depletion line. PKmito Orange FX does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. PKmito Orange FX enables multicolor imaging with SPY505, SPY650, SPY700, SiR or GFP. It can be used for widefield, confocal, SIM or STED imaging in glutaraldehyde fixed cells and tissue. Contains 1 vial of PKmito Orange FX (lyophilized).
