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Rabbit TrueBlot: Anti Rabbit I...

Article
Rabbit TrueBlot: Anti Rabbit IgG HRP
Product Number
18-8816-31
Supplier
Rockland Immunochemicals
Size
50 ul
Description
Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP Anti-Rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
Category
Antibodies
Product Group
Secondary Antibodies
Application
ChIP, ELISA, FISH, IF, IP, WB
Host
Mouse
Species reactivity
Rabbit
Conjugate
Horseradish Peroxidase
Clone ID
eB182
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Shipping Temperature
BLUE ICE
Supplier URL
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