SPY555-tubulin is a bright & non toxic live cell microtubule stain based on SPY™ dyes series. Its optimized structure allows quick labeling of microtubules in live cells with high specificity and very low background. SPY555-tubulin stains microtubules in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission spectra are similar to tetramethylrhodamine (TMR). SPY555-tubulin enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-tubulin can be imaged with standard TMR or Cy3 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living cells and tissue.