SWITCH TO MAIN PAGE
Your cart
  • Your shopping cart is empty!
Subtotal
Go to cart

5-Hydroxymethylcytosine (5-hmC...

Article
5-Hydroxymethylcytosine (5-hmC) antibody (pAb)
Product Number
39791
Supplier
Active Motif
Size
100 ug
Description
Active Motif's 5-Hydroxymethylcytosine (5-hmC) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence, Immunohistochemistry and Methyl DNA Immunoprecipitation, it has been shown to react with Human and Mouse samples, but the sequence is not species specific so it should react with a wide range of sample types.
Category
Antibodies
Product Group
Polyclonal Antibodies
Application
DB, IF, IHC, MeDIP
Host
Rabbit
Species reactivity
Human, Mouse
Formulation
PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Immunogen
This 5-Hydroxymethylcytidine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.
Shipping Temperature
RT
Supplier URL
Visit supplier product page
5-Hydroxymethylcytosine antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 2 μg of 5-Hydroxymethylcytosine antibody (5hmC, maroon bars) or 2 μg of control rabbit IgG (IgG, blue bars). Real time quantitative PCR was performed on the immunoprecipitated DNA and results plotted as % of input DNA. The spiked APC DNA contained either no methylation (DNA), 5-methylcytosine methylation (5-mC) or 5-hydroxymethylcytosine methylation (5-hmC). The 5-hmC antibody shows a 650-fold enrichment for 5-hmC DNA compared to 5-mC DNA or unmethylated DNA.
Size: 100 ug
584.00 CHF

Main Menu

Account

Login