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6X His-tag, AlpHcAbs Rabbit

Article
6X His-tag, AlpHcAbs Rabbit
Product Number
HA721172
Supplier
Huabio
Size
100 ug
Description
His-Tag Antibody is a high quality monoclonal His-Tag antibody (also designated 6X His-Tag antibody or His-Probe antibody) suitable for the detection of the His-Tag protein. His-Tag Antibody is available as both the non-conjugated anti-His-Tag antibody form, as well as multiple conjugated forms of anti-His-Tag antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. Anti-His tag, AlpHcAbs Rabbit antibody is monovalentrecom mbinant single domain antibody fused to rabbit 1gG Fc,and Anti-His tag AlpHcAbs Rabbit antibody detects the His tag selectively,no re eactivity with other proteins.
Category
Antibodies
Product Group
Aptamers & Nanobodies
Application
ELISA, IP, WB
Host
Camelid
Conjugate
Unconjugated
Formulation
PBS
Immunogen
6*His tag fusion protein.
Shipping Temperature
BLUE ICE
Supplier URL
Visit supplier product page
Western blot analysis of 6X His-tag, AlpHcAbs Rabbit on different lysates with Rabbit anti-6X His-tag, AlpHcAbs Rabbit antibody (HA721172) at 1/50,000 dilution. Lane 1: C-terminal His-tag fusion protein lysate Lane 2: N-terminal His-tag fusion protein lysate Lane 3/4/5/6: Mid His-tag fusion protein lysate Lysates/proteins at 50 ng/Lane. Observed band size: 30/45/50 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721172) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Size: 100 ug
470.00 CHF

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