A204 cells are human epithelial cells derived from the muscles of a 1-year-old female patient with rhabdomyosarcoma. With applications in 3D cell culture and tumorigenic properties, A-204 cells provide an opportunity for studying tumour biology and potential therapeutic interventions. Derived from muscle tissue, A-204 cells closely resemble the outer layer of cells found in organs and tissues. The A204 cell line is characterized by its aggressive undifferentiated phenotype, making it a valuable model for investigating the molecular mechanisms of tumorigenesis and metastasis in soft tissue sarcomas. The presence of specific isoenzymes, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3, in A-204 cells provides insight into their metabolic characteristics. These isoenzymes may play a role in understanding cellular processes involved in cancer progression and treatment response. These cells exhibit robust growth in vitro and have been used to study cell proliferation, apoptosis, and drug resistance mechanisms. The A204 cell line is also instrumental in the evaluation of new chemotherapeutic agents and in understanding the interaction between rhabdomyosarcoma cells and therapeutic compounds. This cell line serves as an essential tool for cancer researchers aiming to develop more effective treatments for sarcomas and other related malignancies.
