A curated panel of Glysite™ Explorer lectins covering the major categories of glycan types including mannose, complex N-glycan, core O-glycan, fucose, sialic acid, GalNAc, GlcNAc, and LacNAc has been validated for use with the Glysite™ Explorer in situ PLA Glycan Detection Kit (GEK-1000). Researchers should select a Glysite™ Explorer lectin to pair with a primary antibody of their choice for use with GEK-1000. This provides a simple approach to understand glycan-protein proximity with a spatial context.