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AKT1 Western Chemiluminescent ...

Article
AKT1 Western Chemiluminescent Blotting Kit
Product Number
KCAI51
Supplier
Rockland Immunochemicals
Size
1 kit
Description
Rockland Immunochemicals' Chemiluminescent Western Blot Kit for AKT1 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT1 protein. AKT1 Western Blotting kit allows for the detection of endogenous protein levels of human AKT1 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT1. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT1 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT1 Western Blotting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
Category
Protein Purification & Analysis
Product Group
Electrophoresis Reagents & Kits
Application
ELISA, IF, WB
Host
Mouse
Species reactivity
Human
Conjugate
Unconjugated
Clone ID
14E5.A2.B2.H9
Synonyms
AKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1; Protein kinase B
Unigene ID
207
Gene Symbols
AKT1
Shipping Temperature
DRY ICE
Supplier URL
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