Ab-Match ASSEMBLY Human PAP1 (...
- Article
- Ab-Match ASSEMBLY Human PAP1 (REG3alpha) kit
- Product Number
- 5323
- Supplier
- MBL International
- Size
- 96 tests
- Description
- The anti-PAP1 capture antibody is coated on a 96 microwell plate. Standards or samples are added to the microwells, allowing PAP1 to bind to the capture antibody in proportion to the concentration of PAP1 in the sample (primary reaction). After wash unbound PAP1, biotin conjugated anti-PAP1 detection antibody is added and attaches to the PAP1 bound to coated antibody (secondary reaction). After washing away all unbound detection antibody, peroxidase conjugated streptavidin (SA-HRP) is added and attaches to the biotin label on the bound detection antibody (biotin-avidin reaction). Another wash removes all unbound SA-HRP, before the substrate is added the peroxidase reaction. After adding the stop solution to the microwell plate, the relative amount of PAP1 bound in each well can be determined from the yellow color using a microplate reader. The concentration of PAP1 in each sample can be obtained by comparing the OD of the sample to the OD of the standards on the plate.
- Category
- Protein Purification & Analysis
- Product Group
- Protein & Organelle Purification
- Application
- ELISA
- Synonyms
- hepatocarcinoma-intestine-pancreas; hepatointestinal pancreatic protein; HIP; HIP/PAP; human proislet peptide; INGAP; pancreatic beta cell growth factor; pancreatitis-associated protein 1; PAP; PAP homologous protein; PAP1; PAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; reg III-alpha; REG3; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; regenerating islet-derived protein III-alpha; REG-III; P; REG; reg III-beta; REG-3-beta; regenerating islet-derived protein 3-beta; regenerating islet-derived protein III-beta; RegIII (beta)
- Unigene ID
- 5068
- Gene Symbols
- REG3A
- Shipping Temperature
- BLUE ICE
- Supplier URL
- Visit supplier product page