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Anti Interleukin-10 IL10 Antib...

Article
Anti Interleukin-10 IL10 Antibody Picoband
Product Number
RP1014-100UG
Supplier
Boster Bio
Size
100 ug
Description
Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1014. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Application
ELISA, IHC, WB
Host
Rabbit
Species reactivity
Human
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
E. coli-derived human IL-10 recombinant protein (Position: S19-N178).
Molecular Weight
21 kDa
Synonyms
CSIF; cytokine synthesis inhibitory factor; GVHDS; IL-10; IL10A; interleukin-10; T-cell growth inhibitory factor; TGIF
Unigene ID
3586
Gene Symbols
IL10
Shipping Temperature
BLUE ICE
Western blot analysis of IL10 using anti-IL10 antibody (RP1014). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human IL10 protein 10 ng,<br> Lane 2: recombinant human IL10 protein 5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (RP1014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa.
Size: 100 ug
488.00 CHF

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