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Anti TNF alpha Antibody Picoba...

Article
Anti TNF alpha Antibody Picoband
Product Number
A00002-2-100UG
Supplier
Boster Bio
Size
100 ug
Description
Boster Bio Anti-TNF alpha Antibody Picoband® catalog # A00002-2. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Host
Rabbit
Species reactivity
Mouse
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse TNF alpha, different from the related human sequence by five amino acids, and from the related rat sequence by three amino acids.
Molecular Weight
25896 MW
Synonyms
cachectin; DI; DIF; Tn; TNF-; Tnfa; TNF-a; TNFalpha; TNF-alpha; Tnfs; Tnfsf1a; TNFSF2; Tnlg1f; tumor necrosis factor; tumor necrosis factor ligand 1f; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Unigene ID
21926
Gene Symbols
Tnf
Shipping Temperature
BLUE ICE
Estimated Delivery Time:
14 Business Days
Mediadatasheet
Download datasheet
<b> Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-2). </b><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse thymus tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-2) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.
Size: 100 ug
492.00 CHF

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