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Anti TNF alpha Antibody Picoba...

Article
Anti TNF alpha Antibody Picoband
Product Number
A00002-3-100UG
Supplier
Boster Bio
Size
100 ug
Description
Boster Bio Anti-TNF alpha Antibody Picoband® catalog # A00002-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Application
ELISA, WB
Host
Rabbit
Species reactivity
Human
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
E. coli-derived human TNF alpha recombinant protein (Position: V77-L233).
Molecular Weight
25896 MW
Synonyms
APC1 protein; DIF; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNF-alpha; TNFSF2; TNLG1F; tumor necrosis factor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; Cachectin
Unigene ID
7124
Gene Symbols
TNF
Shipping Temperature
BLUE ICE
Estimated Delivery Time:
14 Business Days
Mediadatasheet
Download datasheet
Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human TNF alpha protein 10 ng,<br> Lane 2: recombinant human TNF alpha protein 5 ng,<br> Lane 3: recombinant human TNF alpha protein 1 ng,<br> Lane 4: recombinant human TNF alpha protein 0.5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (A00002-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17 kDa.
Size: 100 ug
492.00 CHF

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