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Anti TNF alpha Antibody Picoba...

Article
Anti TNF alpha Antibody Picoband
Product Number
A00002-4-100UG
Supplier
Boster Bio
Size
100 ug
Description
Boster Bio Anti-TNF alpha Antibody Picoband® catalog # A00002-4. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Host
Rabbit
Species reactivity
Mouse
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse TNF alpha, which shares 92.3% and 86.7% amino acid (aa) sequence identity with human and rat TNF alpha, respectively.
Molecular Weight
25896 MW
Synonyms
cachectin; DI; DIF; Tn; TNF-; Tnfa; TNF-a; TNFalpha; TNF-alpha; Tnfs; Tnfsf1a; TNFSF2; Tnlg1f; tumor necrosis factor; tumor necrosis factor ligand 1f; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Unigene ID
21926
Gene Symbols
Tnf
Shipping Temperature
BLUE ICE
Estimated Delivery Time:
14 Business Days
Mediadatasheet
Download datasheet
Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-4). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates&#44; <br> Lane 2: mouse thymus tissue lysates&#44; <br> Lane 3: mouse liver tissue lysates&#44; <br> Lane 4: mouse lung tissue lysates&#44; <br> Lane 5: mouse kidney tissue lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-4) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.
Size: 100 ug
492.00 CHF

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