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Anti TP53 Antibody Picoband

Article
Anti TP53 Antibody Picoband
Product Number
A00001-2-10UG
Supplier
Boster Bio
Size
10 ug
Description
Boster Bio Anti-TP53 Antibody Picoband® catalog # A00001-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Application
ELISA, FC, IHC, WB
Host
Rabbit
Species reactivity
Human
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
E.coli-derived human TP53 recombinant protein (Position: M1-D186).
Molecular Weight
53 kDa
Synonyms
antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53; Tumor suppressor p53
Unigene ID
7157
Gene Symbols
TP53
Shipping Temperature
BLUE ICE
Figure 1. Western blot analysis of TP53 using anti-TP53 antibody (A00001-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human T-47D whole cell lysates, <br> Lane 3: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 antigen affinity purified polyclonal antibody (Catalog # A00001-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53 at approximately 53 kDa. The expected band size for TP53 is at 44 kDa.
Size: 10 ug
175.00 CHF

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