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Anti TP53 Antibody Picoband

Article
Anti TP53 Antibody Picoband
Product Number
A00001-2-DYLIGHT594-100UG
Supplier
Boster Bio
Size
100 ug
Description
Boster Bio Anti-TP53 Antibody Picoband® catalog # A00001-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Application
ELISA, FC, IHC, WB
Host
Rabbit
Species reactivity
Human
Conjugate
Red Emitters (615-629nm)
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
E.coli-derived human TP53 recombinant protein (Position: M1-D186).
Molecular Weight
53 kDa
Synonyms
antigen NY-CO-13; BCC7; BMFS5; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; tumor supressor p53; Tumor suppressor p53
Unigene ID
7157
Gene Symbols
TP53
Shipping Temperature
BLUE ICE
Figure 1. Western blot analysis of TP53 using anti-TP53 antibody (A00001-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human T-47D whole cell lysates, <br> Lane 3: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 antigen affinity purified polyclonal antibody (Catalog # A00001-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53 at approximately 53 kDa. The expected band size for TP53 is at 44 kDa.
Size: 100 ug
719.00 CHF

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