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Anti XPC Antibody Picoband

Article
Anti XPC Antibody Picoband
Product Number
A00473-1-BIOTIN-100UG
Supplier
Boster Bio
Size
100 ug
Description
Boster Bio Anti-XPC Antibody Picoband® catalog # A00473-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Application
ELISA, FC, ICC, IF, WB
Host
Rabbit
Species reactivity
Human, Mouse, Rat
Conjugate
Biotin
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
E.coli-derived human XPC recombinant protein (Position: D146-E838).
Molecular Weight
33477 MW
Synonyms
DNA repair protein complementing XP-C cells; mutant xeroderma pigmentosum group C; p125; RAD4; xeroderma pigmentosum, complementation group C; XP3; XPCC; Xeroderma pigmentosum group C-complementing protein
Unigene ID
7508
Gene Symbols
XPC
Shipping Temperature
BLUE ICE
Western blot analysis of XPC using anti-XPC antibody (A00473-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human MCF-7whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: mouse lung tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPC antigen affinity purified polyclonal antibody (Catalog # A00473-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPC at approximately 106KD. The expected band size for XPC is at 106KD.
Size: 100 ug
719.00 CHF

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