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Anti mTOR/MTOR Antibody Picoba...

Article
Anti mTOR/MTOR Antibody Picoband
Product Number
A00003-2-100UG
Supplier
Boster Bio
Size
100 ug
Description
Boster Bio Anti-mTOR/MTOR Antibody Picoband® catalog # A00003-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Category
Antibodies
Product Group
Polyclonal Antibodies
Application
ELISA
Host
Rabbit
Species reactivity
Human, Non-human Primate
Purity
Affinity Purified
Formulation
Lyophilized
Immunogen
E.coli-derived human mTOR/MTOR recombinant protein (Position: N2093-N2537).
Molecular Weight
25896 MW
Synonyms
FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FRAP; FRAP1; FRAP2; mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR; SKS; FKBP12-rapamycin complex-associated protein; Mechanistic target of rapamycin
Unigene ID
2475
Gene Symbols
MTOR
Shipping Temperature
BLUE ICE
Estimated Delivery Time:
14 Business Days
Mediadatasheet
Download datasheet
Western blot analysis of MTOR using anti-MTOR antibody (A00003-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: monkey COS-7 whole cell lysates, <br> Lane 5: human A431 whole cell lysates, <br> Lane 6: human Jurkat whole cell ltsates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR antigen affinity purified polyclonal antibody (Catalog # A00003-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
Size: 100 ug
492.00 CHF

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