ChIP-IT Express Enzymatic
- Article
- ChIP-IT Express Enzymatic
- Product Number
- 53009
- Supplier
- Active Motif
- Size
- 25 reactions
- Description
- ChIP-IT Kits utilize protein G-coated magnetic beads instead of traditional agarose beads, making it possible to perform chromatin immunoprecipitation (ChIP) in just 1 day. The ChIP-IT Express method improves on traditional ChIP by reducing or eliminating several time-consuming steps. Because the supplied magnetic beads have much lower background than traditional agarose beads, pre-clearing and blocking steps are no longer necessary. Washing is easier because the spin steps have been replaced by rapid magnetic pull-down. And, only 3 washes are required, as compared to 8 washes with traditional ChIP protocols. This dramatically reduces the amount of hands-on time during the assay. This not only saves time, but reducing the number of times the sample is manipulated minimizes DNA losses that can occur during wash steps, which helps improve yield as well as sample-to-sample consistency. The ChIP-IT Express Kit uses sonication for preparing the sheared chromatin for use in ChIP, while ChIP-IT Express Enzymatic features a more user-friendly method to shear the chromatin by enzymatic digestion, which helps eliminate some of the complications that can be caused by sonication, such as emulsification and overheating. Both kits feature fast, reproducible protocols that make ChIP more successful while reducing your time and effort.
- Category
- Assay Kits
- Product Group
- Cellular Assays
- Application
- ChIP
- Shipping Temperature
- DRY ICE
- Estimated Delivery Time:
- 5 Business Days
- Supplier URL
- Visit supplier product page