ChIP-IT Express Enzymatic Shea...
- Article
- ChIP-IT Express Enzymatic Shearing Kit (included in 53009)
- Product Number
- 53035
- Supplier
- Active Motif
- Size
- 10 reactions
- Description
- The first step in successful ChIP is shearing the chromatin into 200-1000 bp fragments. Active Motif offers two different methods for shearing chromatin. The more traditional method for shearing involves subjecting the isolated chromatin to repeated pulses of ultrasonic waves that are produced by a sonicator. The reagents included in the ChIP-IT Shearing Kit (Catalog No. 53032) can be used with Active Motif's EpiShear Probe Sonicator (Catalog No. 53051) to prepare your chromatin samples for use with our ChIP-IT Express Kits, as well as with other ChIP methods. Although sonication is an effective method for shearing DNA, not everybody owns a sonicator or wishes to become proficient in its use. For new users, it can be difficult to optimize sonication parameters and to get reproducible results due to complications that can arise during sonication, such as emulsification and overheating of the sample. Because of this, Active Motif has developed a robust and extremely user-friendly method to shear chromatin for ChIP by digesting it. The ChIP-IT Enzymatic Shearing Kit (Catalog No. 53035) uses a proprietary Enzymatic Shearing Cocktail that quickly shears DNA into 200-1000 bp fragments. Because enzymatic shearing is solely time and temperature dependent, the problems associated with sonication are eliminated. Enzymatic shearing is a good choice for users who do not do not plan to do enough ChIP to make it worthwhile to invest the time and money required to buy and learn how to use a sonicator.
- Category
- Protein Purification & Analysis
- Product Group
- Protein & Organelle Purification
- Application
- ChIP
- Shipping Temperature
- DRY ICE
- Supplier URL
- Visit supplier product page