Active Motif's ChIP-IT PBMC Kit overcomes the challenges of generating quality chromatin for use in ChIP from peripheral blood mononuclear cells (PBMCs). PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells, constitute a critical component of the peripheral immune system and are often used in immunology, cancer and infectious disease research. However, for researchers who want to perform ChIP, PBMCs make it difficult to extract quality chromatin because they are highly resistant to lysis under conditions normally suitable for other cell types. Active Motif leveraged its years of experience working with these difficult-to-lyse cells into developing a kit to finally enable researchers to prepare chromatin and perform ChIP on PBMCs.
