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Chemiluminescent Western Blot ...

Article
Chemiluminescent Western Blot Kit for use with Goat Primary Antibody FemtoMax
Product Number
KCA004
Supplier
Rockland Immunochemicals
Size
1 kit
Description
This Chemiluminescent Western Blot Kit allows for the detection of primary Goat polyclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of our FemtoMax Chemiluminescent Substrate Kit for Western Blotting, primary and secondary antibodies can be used at greater dilutions. If you currently dilute your primary antibody 1:1,000 using an ECL substrate equivalent, then dilute your antibody 1:5,000 using the FemtoMax substrate.
Category
Protein Purification & Analysis
Product Group
Electrophoresis Reagents & Kits
Application
WB
Species reactivity
Goat
Conjugate
Unconjugated
Shipping Temperature
BLUE ICE
Supplier URL
Visit supplier product page
Chemiluminescent Western Blot Kit shows super sensitive signal. Known amounts of GST were spiked into a HeLa lysate followed by separation by SDS-PAGE using a 4-20 % gradient gel. Proteins were transferred to a Protran B85 membrane for 1 hour at 100 mV. The membrane was blocked with 5% BLOTTO (p/n B501-0500) in TTBS for 1 h at 4 °C. The blot was probed for 40 min with 1:1,000 dilution of Mab anti-GST (p/n 200-301-200) followed by HRP anti-Mouse IgG (p/n 610-103-121) at 1:10,000 dilution in blocking buffer for 1 h at 4°C.
Size: 1 kit
463.00 CHF

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