For simple, high efficiency E. coli transformations without heat shock and lengthy incubations.|The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go!</i? E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium ,ai Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/ug pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.