PD-1/IL-2 Luciferase Reporter ...
- Article
- PD-1/IL-2 Luciferase Reporter Jurkat Cell Line
- Product Number
- 82117
- Supplier
- BPS Bioscience
- Size
- 2 vials
- Description
- PD-1/IL-2 Luciferase Reporter Jurkat Cell Line is a Jurkat T cell line constitutively expressing human PD-1 (Programmed Cell Death 1, also known as PDCD1, SLEB2, CD279, GenBank Accession #NM_005018), and conditionally expressing firefly luciferase under the control of a human interleukin-2 (IL-2) promoter. This cell line has been validated by flow cytometry for expression of PD-1, and in co-culture assays that assess the PD-L1/PD-1 interaction on Jurkat T cell activation. Figure 1: Illustration of the mechanism of action of PD-1/IL-2 Luciferase Reporter Jurkat Cell Line in a co-culture assay. The TCR activator presented at the surface of PD-L1/TCR Activator CHO cells stimulates TCR (T cell receptor) in Jurkat T cells, whereas overexpression of PD-L1 on the CHO cell line engages Jurkat PD-1 resulting in blocking TCR signaling and preventing activation of the IL-2 promoter. Addition of a neutralizing anti-PD-1 or anti-PD-L1 antibody to the co-culture prevents formation of the PD-L1/PD-1 complex, and results in TCR activation and increased IL-2 promoter activity, which translates into increased luciferase reporter signal.
- Category
- Cell Culture
- Product Group
- Primary Cells & Cell Lines
- Application
- Neut
- Synonyms
- aldesleukin; IL-2; interleukin-2; involved in regulation of T-cell clonal expansion; lymphokine; T cell growth factor; TCGF; T-cell growth factor
- Unigene ID
- 3558
- Gene Symbols
- IL2
- Shipping Temperature
- DRY ICE
- Supplier URL
- Visit supplier product page
Size: 2 vials
price on request