PD-1 CRISPR/Cas9 Lentivirus (I...
- Article
- PD-1 CRISPR/Cas9 Lentivirus (Integrating)
- Product Number
- 78052
- Supplier
- BPS Bioscience
- Size
- 2 x 500 ul
- Description
- The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found on most cancers, and the PD-1:PD-L1/2 interaction inhibits T-cell activity and enables cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes. The PD-1 CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 4 sgRNA (single guide RNA) targeting human PD-1 (Programmed Cell Death 1, PDCD1, CD279, GenBank Accession #NM_005018) driven by a U6 promoter (Figures 1 and 2). The integrating lentivirus integrates randomly into the cell's genome to express both the Cas9 and sgRNA. Puromycin selection increases the knockout efficiency by forcing high expression levels of both Cas9 and the sgRNA, and can be used with the integrating lentivirus to quickly and easily achieve high knockdown efficiencies in a cell pool. Efficiencies also depend on the cell type and the gene of interest.
- Category
- Cell Culture
- Product Group
- Primary Cells & Cell Lines
- Formulation
- The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
- Shipping Temperature
- DRY ICE
- Supplier URL
- Visit supplier product page
Size: 2 x 500 ul
966.00 CHF