The Re-ChIP-IT Kit utilizes the efficient magnetic bead-based protocol used in Active Motif's popular ChIP-IT Express Kits to provide a convenient kit for Re-ChIP, which is also known as Sequential ChIP, Chromatin Re-IP and ChIP Re-ChIP. Re-ChIP is a method in which two sequential chromatin immunoprecipitations are performed using two different antibodies. This makes it possible to assay for the simultaneous presence of two proteins or distinct histone modifications at the same genomic region of interest. The Re-ChIP-IT Kit works with chromatin prepared using either sonication or enzymatic shearing, is compatible with multi-channel pipetting and requires fewer cells than other methods.
