Recombinant Histone H3K4me3 bi...
- Article
- Recombinant Histone H3K4me3 biotinylated (EPL)
- Product Number
- 31282
- Supplier
- Active Motif
- Size
- 25 ug
- Description
- Recombinant Histone H3 trimethyl Lys9 (H3K9me3) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing trimethyl lysine 9 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,268 Daltons. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant histones are used to analyze histone modifications, or to generate chromatin in vitro.
- Category
- Proteins, Peptides and Small Molecules
- Product Group
- Proteins
- Conjugate
- Biotin
- Purity
- >98% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry
- Formulation
- 25 ug supplied at 25 as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
- Molecular Weight
- 15,268 Daltons
- Shipping Temperature
- DRY ICE
- Supplier URL
- Visit supplier product page
Size: 25 ug
622.00 CHF