Recombinant Sortase A5 protein
- Article
- Recombinant Sortase A5 protein
- Product Number
- 13101
- Supplier
- Active Motif
- Size
- 250 ug
- Description
- Sortag-IT Labeling Kits are designed for site-specific labeling of Active Motif's highly specific AbFlex recombinant antibodies (rAb) via the Sortase tag recognition sequence (LPXTG) that is incorporated into the heavy chains of each AbFlex antibody. Directly conjugate fluorophores, enzymes (HRP, AP), biotin, peptides, DNA, carbohydrates or other labels to the terminal end of the heavy chain to ensure the antigen binding site remains available for target recognition. The Sortag-IT Labeling Kits use Active Motif's Sortase A5 pentamutant from Staphylococcus aureus that is significantly more active than wild-type Sortase to provide a faster, more efficient labeling reaction. Simply add your AbFlex antibody with the poly-Glycine label, add Sortase A5 and incubate for approximately 1 hour. Purification columns are included to remove excess label and Stop Solution is provided to inactivate the Sortase A5 enzyme. Labeled antibodies are ready for downstream analysis or can be stored at 4C for up to 3 months.
- Category
- Proteins, Peptides and Small Molecules
- Product Group
- Proteins
- Formulation
- Sortase A5 protein expressed in E. coli and provided at 1 mg/ml in 50 mM HEPES pH 7.5, 150 mM NaCl and 20% glycerol. Sortase A5 protein labeling set - 50 ug (Cat. No. 13100), includes:Sortase A5 - 50 ug Reaction Buffer - 1 mlStop Solution - 150 ul Sortase A5 protein labeling kit - 250 ug (Cat. No. 13101), includes: Sortase A5 - 250 ug Reaction Buffer - 5 x 1 ml Stop Solution - 5 x 150 ul
- Molecular Weight
- 17.8 kDa
- Shipping Temperature
- DRY ICE
- Supplier URL
- Visit supplier product page
Size: 250 ug
1'818.00 CHF