Recombinant c-Jun protein
- Article
- Recombinant c-Jun protein
- Product Number
- 31116
- Supplier
- Active Motif
- Size
- 5 ug
- Description
- Full-length recombinant p53 protein was expressed in a baculovirus system with a His-Tag and purified by an affinity column in combination with FPLC chromatography. Active Motif also offers Recombinant p53 protein (Catalog No. 31465) that was expressed in E. coli. p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and >NFkB p65), both of which serve to inhibit apoptosis. Recombinant p53 is used in TransAM, DNA-protein (gelshift) and protein-protein interaction assays.
- Category
- Proteins, Peptides and Small Molecules
- Product Group
- Kinases & Other Enzymes
- Formulation
- 5 ug of Recombinant p53protein in Dilution Buffer AM1 (20 mM Tris-Cl (pH 7.5), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA).
- Shipping Temperature
- DRY ICE
- Supplier URL
- Visit supplier product page
Size: 5 ug
651.00 CHF