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Recombinant p300 (1041-1161) p...

Article
Recombinant p300 (1041-1161) protein
Product Number
31372
Supplier
Active Motif
Size
100 ug
Description
The peptide corresponding to amino acids 959-1059 that contains the bromodomain sequences of TRIM33 (accession number NP_056990.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 17.7 kDa. Tripartite motif-containing 33 (TRIM33) protein, also known as TIF1g, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM33 is an E3 ubiquitin protein ligase that promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. TRIM33 does not affect SMAD4 levels but rather acts as an inhibitor of the SMAD4-dependent TGFb/BMP signaling cascade by monoubiquitinating SMAD4 and hampering its ability to form a stable complex with activated SMAD2/3. TRIM33 may also act as a transcriptional corepressor. It has also been shown to associate with SMAD2 and SMAD3 and stimulate differentiation of hematopoietic stem cells. Additionally, TRIM33 associates with TRIM24 and plays a role in the control of cell proliferation. Recombinant TRIM33 (959-1069) can be used in binding assays and inhibitor screening.
Category
Proteins, Peptides and Small Molecules
Product Group
Kinases & Other Enzymes
Purity
>90%
Formulation
Recombinant TRIM33 (959-1069) protein expressed in E. coli and supplied in 25mM Tris-HCl 7.4, 300 mM NaCl, 5% glycerol.
Molecular Weight
17.7 kDa
Shipping Temperature
DRY ICE
Supplier URL
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