This 3,3'5,5'- tetramethylbenzidine (TMB) is an insoluble chromogen substrate for horseradish peroxidase detection systems for IHC, ICC and In Situ hybridization. This is ready to use one component system and gives aqua brilliant blue color with peroxidase. Neutral Red color can be used as a counterstain. This TMB solution contains TMB, 1.25 mMol L-1, Hydrogen Peroxide substrate, acetate buffer, pH 4.9, non-toxic proprietary stabilizers and precipitating agents.