Active Motif's Tag-ChIP-IT Kit provides a method to perform ChIP without the need for protein-specific antibodies. Simply create an expression construct containing your protein of interest in-frame with a C-terminal AM-tag, which has been designed to produce highly consistent and reliable ChIP results. The method is ideal when working with targets that don't have ChIP antibodies available, or only low affinity antibodies, and it enables you to study isoforms, mutations and truncations. Active Motif's AM-tag offers several advantages over other tags, such as FLAG, GFP and HA. AM-tag is specifically designed to give low background signal in ChIP experiments. Also, in contrast to larger GFP and Halo tags, the low molecular weight and unstructured design of the AM-tag reduces the possibility of impeding on the DNA binding domain. In addition, we have designed a high specificity antibody to the tag to increase the pull down efficiency during immunoprecipitation.
