Non ionic detergents like Triton X-100 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background.Triton X-100 (polyethyleneglycolp (1,1,3,3 tetramethylbutyl) phenylether ,octylphenolethoxylate, polyoxyethylene octyl phenyl ether,4-octylphenol polyethoxylate,Mono 30, TX-100, t-octylphenoxypolyethoxyethanol, Octoxynol-9it lower surface tension of water. Triton X-100 is also used for recovery of membrane components under mild non-denaturing conditions.
Category
Lab Reagents & Chemicals
Product Group
Biochemicals
Application
ELISA, ICC, IF, IHC, WB
Formulation
This detergent is supplied as 25% in water, 0.05% ProClin 300.
