An ionizable cationic lipid (pKa 6.09), has been used in the generation of LNPs for the delivery of mRNA, siRNA, and plasmid DNA in vitro and in vivo, LNPs containing ALC-0315 and encapsulating siRNA targeting Factor VII mRNA decrease Factor VII mRNA levels in hepatocytes in mice, administration of SARS-CoV-2 mRNA in ALC-0315-containing LNPs induces the production of IgG that binds to the SARS-CoV-2 BD) in rhesus macaques, with a boost in antigen-specific IgG GMTs seven and 14 days after a second dose, LNPs containing ALC-0315 and encapsulating ovalbumin mRNA reduce tumor volume in an ovalbumin-expressing B16/F10 murine melanoma model
