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BCA Protein Quantification Kit

Article
BCA Protein Quantification Kit
Product Number
C0050-2000TESTS
Supplier
TargetMol
Size
2000 tests
Description
The Bicinchoninic acid (BCA) assay is a stable, sensitive and highly compatible method for the determination of protein concentration. It is commonly used for quantifying the total amount of proteins after extraction. The detection mechanism of BCA assay involves two reations. The first reation is based on the biruet reaction, in which the protein chelates with copper (II) ion in an alkaline environment and reduces the copper (II) to copper (I), forming a blue complex. The second reaction is the chelation of BCA with the cuprous ion, resulting in an intense purple color. The purple colored reaction product is formed by the chelation of two molecules of BCA with one cuprous ion. The complex exhibits a strong linear absorbance at 562 nm with increasing protein concentrations.
Category
Protein Purification & Analysis
Product Group
Western Blotting Reagents
Shipping Temperature
BLUE ICE
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