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Brain-derived neurotrophic fac...

Article
Brain-derived neurotrophic factor, pro- (proBDNF), Human, Mouse, Rat, Rapid ELISA assay
Product Number
BEK-2217-2P
Supplier
Biosensis
Size
2 plate kit
Description
The Biosensis proBDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Category
Assay Kits
Product Group
ELISA Kits
Application
ELISA
Species reactivity
Human, Mouse, Rat
Immunogen
Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Synonyms
abrineurin; ANON2; brain-derived neurotrophic factor; BULN2; neurotrophin
Unigene ID
627
Gene Symbols
BDNF
Shipping Temperature
BLUE ICE
Supplier URL
Visit supplier product page

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