GFP Tag Antibody

Article: GFP Tag Antibody
Product Number: AM1009A-EV
Supplier: Abcepta
Size: 50 ul
Category: Antibodies
Product Group: Monoclonal Antibodies
Application: IF, WB
Host: Mouse
Species reactivity: Human
Clone ID: 168AT1211
Molecular Weight: 27 k
Shipping Temperature: BLUE ICE
Supplier URL: Visit supplier product page

Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela(human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0. 1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody (Product # AM1009a) at a dilution of 1:25 for 1 hour at 37C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody (Product # 1511348) at a dilution of 1:200 for 60 minutes at 37℃. The nuclear counter stain is DAPI (blue).

200 ul
50 ul

Size: 50 ul

220.00 CHF