HumanKine recombinant human No...

Article: HumanKine recombinant human Noggin protein- GMP grade
Product Number: HZ-1118-GMP-100UG
Supplier: Proteintech
Size: 100 ug
Category: Proteins, Peptides and Small Molecules
Product Group: Proteins
Species reactivity: Human, Mouse
Purity: >95%
Formulation: 1x PBS, See Certificate of Analysis for details
Molecular Weight: 65 kDa, homodimer, glycosylated
Synonyms: noggin; SYM1; symphalangism 1 (proximal); SYNS1; SYNS1A
Unigene ID: 9241
Gene Symbols: NOG
Shipping Temperature: RT
Supplier URL: Visit supplier product page

Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 3-15 ng/mL.

Size: 100 ug

1'105.00 CHF