PI(4)P PIP BeadsTM are composed of agarose with 10 nanomoles of bound PI(4)P per mL of beads, enough for several protein binding experiments. Each 1 mL order of PIP Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase. \n \n \n \nStorage \n4 °C - do not freeze \nApplications \nPIP BeadsTM are designed for use in protein pull-down experiments to identify and characterize phosphoinositide binding proteins. Possible applications include the isolation of PIP-binding proteins present in a cell lysate or in a mixture of in vitro translated peptides, for separation by SDS-PAGE and further analysis. \nFeatured in Publication \n1) Casamayor, A. and M. Snyder (2003). "Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function." Mol Cell Biol 23(8): 2762-77. \n2) Hu, Y., Z. Liu, et al. (2005). "Phosphoinositol lipids bind to phosphatidylinositol 3 (PI3)-kinase enhancer GTPase and mediate its stimulatory effect on PI3-kinase and Akt signalings." Proc Natl Acad Sci U S A 102(46): 16853-8. \n3) Brombacher, E., S. Urwyler, et al. (2009). "Rab1 guanine nucleotide exchange factor SidM is a major phosphatidylinositol 4-phosphate-binding effector protein of Legionella pneumophila." J Biol Chem 284(8): 4846-56. \n4) Galvez-Santisteban, M., Rodriguez-Fraticelli, A. and Martin-Belmonte, F. (2014). Phosphoinositides Coated Beads Binding Assay. Bio-protocol 4(3): e1039. \n5) Jiang, K., et al. (2016). "PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail." PLoS Biol 14(2): e1002375. \n \nBeing bio-reactive blog: "Lipid-Ligand interaction tools: lipids are not scary intermediates anymore" \n \n \n \nDocuments \nTechnical Data Sheet
